Kayayyaki
HEPES sodium gishiri
Makamantu: HEPES sodium gishiri; 75277-39-3; HEPES hemisodium gishiri; 103404-87-1; sodium 2- (4- (2-hydroxyethyl) piperazin-1-yl) ethanesulfonate; sodium 2-[4- (2-hydroxyethyl). Piperazin-1-yl] etanesulfonate; HEPES (sodium); C8H17N2NaO4S; 4- (2-Hydroxyethyl) piperazine-1-ethanesulfonic acid hemisodium gishiri; 1-Piperazineethanesulfonic acid, 4- (2-hydroxyethyl) -, monosodium gishiri; 4- (2-Hydroxyethyl) piperazine-1- ethanesulfonic acid sodium gishiri; 4- (2-hydroxyethyl) piperazin-1-ylethanesulphonate, UNII-Z9FTO91O8A; Z9FTO91O8A; sodium; 2- [4- (2-hydroxyethyl) piperazin-1-yl] ethanesulfonate; DTXSID4044458; CHEBI: 4675 278-169-7. gishiri,> = 99.5% (titration); sodium hepes; N- (2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid) sodium gishiri; MFCD00036463; HEPES, sodium gishiri; HEPES sodium gishiri, 98%. (1M) daraja;AKOS015897769;AKOS015912229;AKOS015964204;NCGC00255791-01;CAS-75277-39-3;HY-108535;CS-0029103;FT-06108Asodium; N'-hydroxyethyl-N-piperazineethanesulfonate; HEPES sodium gishiri, BioXtra,> = 99.0% (titration); HEPES sodium gishiri, Vetec (TM) reagent sa, 96%; W-104397; Q27120698; Sodium2- (4- (2-hydroxyethyl) piperazin-1-yl) ethanesulfonate; 1-piperazineethanesulfonic acid, monosodium gishiri; N- (2-Hydroxyethyl) piperazine-N'- (2-ethanesulfonic acid) sodium gishiri; N-2-Hydroxyethylpiperazine-N'-2-ethanesulphonic acid hemisodium gishiri, HEPES sodium gishiri, BioPerformance Certified, dace da cell al'ada, >> 99.5%
Abubuwan Sinadarai na HEPES Sodium Salt
● Bayyanar / Launi: farin foda
● Ruwan tururi: 0Pa a 25 ℃
● Matsayin narkewa: 234 °C
● PKA: 7.5 (a 25 ℃)
● PSA:92.29000
Yawan yawa: 1.504 [a 20 ℃]
● LogP: -0.90190
● Ajiya Temp.: Adana a RT.
● M.: Hygroscopic
● Solubility.: H2O: 1 M a 20 ° C, bayyananne, mara launi
● Ruwan Solubility.: Yana narkewa cikin ruwa.
● Ƙididdigar Ƙididdiga na Ƙididdiga na Hydrogen: 1
● Ƙididdiga Mai Karɓar Haɗin Ruwa na Hydrogen: 6
● Ƙididdiga Mai Juyawa: 5
● Daidaitaccen Mass: 260.08067248
● Ƙididdigar Atom mai nauyi:16
● Matsala:272
Bayanin Tsaro
● Hoton(s):
● Lambobin haɗari:
● Bayanan Tsaro: 22-24/25
Mai amfani
Azuzuwan sinadarai:Sauran Amfani -> Abubuwan Buffer Halittu
MURMUSHI na Canonical:C1CN(CCN1CCO)CCS(=O)(=O)[O-].[Na+]
Amfani:Maganin gishiri na HEPES ya dace da nazarin al'adun tantanin halitta. Ana iya amfani da shi wajen shirya maganin buffer na HEPES. Ana iya amfani da shi azaman mai ɗaukar hoto yayin gwajin phosphorylation a cikin fibroblasts masu lalacewa. Ana iya amfani da shi don tsara hadadden buffer dialysis, wanda aka yi amfani da shi wajen tantance thyroxin kyauta a cikin magani ta radioimmunoassay. Maganin gishiri na HEPES ya dace don amfani a cikin nazarin al'adun tantanin halitta. Ana iya amfani da shi wajen shirya maganin buffer na HEPES. Ana iya amfani da shi azaman mai ɗaukar hoto yayin gwajin phosphorylation a cikin fibroblasts masu lalacewa. Ana iya amfani da shi don tsara hadadden buffer dialysis, wanda aka yi amfani da shi wajen tantance thyroxin kyauta a cikin magani ta radioimmunoassay.
Cikakken Gabatarwa
HEPES sodium gishiri, wanda kuma aka sani da 4- (2-hydroxyethyl) piperazine-1-ethanesulfonic acid sodium gishiri, wani nau'i ne na HEPES da aka saba amfani dashi. Wani fili ne na kwayoyin halitta na zwitterionic wanda ke aiki a matsayin wakili na buffering a binciken nazarin halittu da kwayoyin halitta.
HEPES sodium gishiri ana amfani dashi sau da yawa a cikin kafofin watsa labarai na al'adar tantanin halitta da masu buffer nazarin halittu saboda ikonsa na kiyaye tsayayyen kewayon pH a kusa da yanayin physiological (pH 7.2 - 7.6). Yana da narkewa sosai a cikin ruwa kuma yana da ƙarancin ƙarancin guba, yana sa ya dace da aikace-aikace daban-daban.
An fi son nau'in gishiri na sodium na HEPES a yawancin aikace-aikace kamar yadda ya inganta solubility da kwanciyar hankali, idan aka kwatanta da nau'in acid kyauta. Ana samuwa a kasuwa a matsayin farin crystalline foda wanda za'a iya narkar da shi cikin sauƙi a cikin ruwa don shirya mafita na aiki.
Masu bincike galibi suna amfani da gishirin sodium na HEPES a cikin dabarun ilimin ƙwayoyin cuta, al'adar tantanin halitta, ƙididdigar enzyme, tsarkakewar furotin, da sauran gwaje-gwajen sinadarai inda sarrafa pH ke da mahimmanci. Ƙarfin ajiyarsa da dacewa tare da tsarin halittu sun sa ya zama sanannen zaɓi don kiyaye mafi kyawun yanayi don aiki da kwanciyar hankali na ƙwayoyin halitta iri-iri.
Kafin amfani da gishiri sodium HEPES a cikin kowane takamaiman aikace-aikacen, yana da mahimmanci don nazarin wallafe-wallafen, tuntuɓi shawarwarin masu kaya, da haɓaka ƙaddamarwa da kewayon pH dangane da takamaiman buƙatun gwaji.
Aikace-aikace
HEPES sodium gishiri yana samun aikace-aikacen tartsatsi a wurare daban-daban na nazarin halittu da nazarin halittu. Wasu daga cikin mahimman amfaninsa sun haɗa da:
Al'adun Kwayoyin Halitta:HEPES sodium gishiri ana yawan ƙarawa zuwa kafofin watsa labarai na al'ada don kiyaye tsayayyen pH a cikin kewayon ilimin lissafi da samar da yanayi mai dacewa don sel don girma da haɓaka.
Wakilin Buffering:HEPES sodium gishiri ana yawan amfani da shi azaman wakili na buffer a cikin abubuwan buffers nazarin halittu da mafita, gami da ƙididdigar enzyme, tsarkakewar furotin, da dabarun ilimin ƙwayoyin cuta. Yana taimakawa ci gaba da pH ta hanyar tsayayya da canje-canje a cikin acidity ko alkalinity.
Electrophoresis: Ana amfani da gishirin sodium na HEPES azaman buffer a cikin gel electrophoresis don kiyaye tsayayyen pH yayin rabuwar acid nucleic ko sunadarai. Yana hana canje-canje a cikin pH wanda zai iya rinjayar ƙaura da tsarin rabuwa na kwayoyin halitta a cikin gel.
Karfin Halitta: HEPES sodium gishiri ana ƙara zuwa furotin mafita don daidaita tsarin su da kiyaye ayyukansu. Yana taimakawa kula da mafi kyawun yanayin pH da ake buƙata don kwanciyar hankali na furotin da aiki.
Ayyukan Enzyme: Ana amfani da gishirin sodium HEPES azaman buffer a cikin halayen enzymatic don kula da pH da ake so don ingantaccen aikin enzyme. Yana taimakawa tabbatar da cewa enzymes suna aiki a mafi girman inganci da daidaito.
Hoton Tantanin halitta:HEPES gishirin sodium galibi ana haɗa shi cikin kafofin watsa labarai na hoto don gwaje-gwajen hoton tantanin halitta. Ƙarfin buffersa yana taimakawa kula da pH da ake so kuma yana hana sauye-sauye wanda zai iya tasiri ga iyawa da haske na sel.
Dabarun Halittar Halitta: Ana amfani da gishirin sodium na HEPES sosai a cikin dabarun nazarin halittu daban-daban, gami da DNA ko keɓewar RNA, PCR, jerin DNA, da nazarin furotin. Ƙarfin buffer ɗin sa yana ba da damar ingantaccen sakamako mai dogaro yayin waɗannan hanyoyin.
Yana da mahimmanci a lura cewa takamaiman aikace-aikacen da tattarawar gishirin sodium na HEPES na iya bambanta dangane da buƙatun gwaji da tsarin nazarin halittu da ke ƙarƙashin binciken. Don haka, yana da kyau a tuntuɓi wallafe-wallafen da shawarwarin masu samarwa don ingantaccen amfani.
