Hepes gishiri gishiri; CAS A'a.: 75277-39 - hoton da aka buga

Hepes gishiri gishiri; CAS A'a.: 752777-39

A takaice bayanin:

Sunan sunadarai:Hepes sodium gishiri
CAS No.:75277-39-39
CASTRECILated CAS:1159813-57-6
Tsarin kwayoyin halitta:C8h17n2nao4S
Nauyi na kwayoyin:260.29
Lambar HS.:293359995
Lambar Turai (EC):27-16-7,68-394-6
UNII:Z9Fto91o8A
ID na DSSTOX:Dtxsid400444458
Lambar Nikkaji:J307.551F
Wikidata:Q27120698
Chembl id:Chembl3187284
Fayil na Mol:75277-39-3.mol

Cikakken Bayani

Tags samfurin

Kwatanci: Hepes gishiri (sodium); c8h17n2no4s; 4- (2-hyhroxineethonic gishiri; 1-butafine-1- (2-hydroxyethyl) -, monoserium gishiri; 4- (2-hydroxyethyl) butperazine-1-ethanesulfonic gishiri mai sodium; sodium 4- ((2-hydroxyethyl) Piperazin-1-Ylethanesgphonate; Unii-Z9fto91O8A; Z9Fto91o8a; sodium; 2- [4- (2-) (2-hydroxyethyl) bututun-2-yl] ethanitulfonate; dtxsid40444458; Chebi: 46758; einecs 27-159-7; 1-piperzineethonic acid, 4- (2-hyhroxyethyl) bututun giya; nyes sodium gishiri,> = 99.5% (titress); sodium hepes; n- (2-hydroxyethyl) butperazine-n '- (2-ethanesultultfonic gishiri; mfcd0003646; hepes, sodium gishiri, 98%; c8h18n2o4S.Na; ya kasance; ya kasance; (1m); tox21_30155; hb5187; n- (2-ethelyulfulonicacid) bututun ruwa na hemisodium sa; akos0158977777790; Akos015912229; Akos09996101-01; Hy-061086; F238319 N'-hydroxyeethyl-n-buttererazineethanes; hepes sodium gishiri, bixtrra, gishiri ne, da gishiri 96%; W-104397; Q27120698; Sodium2- (4- (2- (2- hydroxneyl) Piperazin-1 -1-m hydroxyeethonate; 4- (2-hydroxyeethonate bututun -1-pipererazineethonic acium gishiri; 4- (2-hydroxyeethyl) -1-pipererazineethonic acid, monoserium gishiri; n-2-hyhroxyethylperazine-n'-2-ethanesulsulsulphonic acid gishiri; Hepes sodium gishiri, bereroforthation na bioopirfuwa, dace da al'adun kwayar halitta,> = 99.5%

Kayan guba na hepes sodium gishiri

Bayyanar / launi: farin foda
● Trapher matsa lamba: 0pa a 25 ℃
Point ● Melting Point ° C
● Poka: 7.5 (a 25 ℃)
● PSA:92.29000
Bensity: 1.504 [A 20 ℃]
● Laja: -0.90190

● Storest Hust. asso a RT.
● Mai hankali .:::yangroscopic
● Sulquility.:h2o: 1 m A 20 ° C, Share, mai launi
● Cikin ruwa mai ruwa.:it yana da narkewa cikin ruwa.
● Hydrogen Bond Donor Kidaya: 1
● Hydrogen Bond Karatun: 6
Kidaya Bond: 5
● daidai taro: 260.08067248
● Kididdige da atom count: 16
Komadan gaba: 272

Bayanin Safya

● Picogram (s):
Lambobin Hare Hatsari:
Bayanin lafiya: 22-24 / 25

M

A Daraktsan Kwarewar:Sauran Amfani -> Buga nazarin halittu
Canonical murmushi:C1CN (CCN1CO) CCS (= o) (= o) [O -]. [Na +]
Amfani:Maganin gishirin sodium maganin sodium ya dace da nazarin al'adun tantanin halitta. Ana iya amfani dashi a cikin shiri na maganin maganin hepes. Ana iya amfani dashi azaman mai buffer a lokacin phosphoryby na phosphoryborized fibroblasts. Ana iya amfani da shi don tsara mai bufaye sankara na dialsis, wanda aka yi amfani da shi a cikin ƙuduri na onumexin kyauta a cikin Serum ta rediyo. Maganin gishirin sodium na gishiri ya dace don amfani a cikin binciken al'adun tantanin halitta. Ana iya amfani dashi a cikin shiri na maganin maganin hepes. Ana iya amfani dashi azaman mai buffer a lokacin phosphoryation har zuwa fibroblasts. Ana iya amfani da shi don tsara mai bufaye sankara na dialsis, wanda aka yi amfani da shi a cikin ƙuduri na onumexin kyauta a cikin Serum ta rediyo.

Cikakken gabatarwar

Hepes sodium gishiri, wanda aka sani da 4-- (2--hydroxyethyl) bututun ruwa-1-ethanesulfonic gishiri mai tsami, sigar da aka saba amfani da shi ne na hepes. Yana da kwayoyin halitta na zuwitterionic wanda ke aiki a matsayin wakili mai gina jiki a cikin binciken halittu da biochemical.
Sau da yawa ana amfani da gishiri na ruwan sodium a al'adun sel watsa allo da kuma abubuwan fashewa saboda iyawarsa ta tsayayyen yanayin (PH 7.2 - 7.6). Yana da matukar narkewa a cikin ruwa kuma yana da ƙarancin guba, sanya ya dace da aikace-aikace iri-iri.
An fi son ƙwayar ƙwayar sodium na hepess a aikace-aikace da yawa yayin da yake haɓaka kayan haɓaka da kwanciyar hankali, idan aka kwatanta da fom ɗin kyauta. Yana da kasuwanci azaman farin lu'ulu wanda za'a iya narkar da narkar da ruwa cikin ruwa don shirya mafita aiki.
Masu bincike yawanci suna amfani da gishiri na gishirin sodium a cikin dabarun kwayar halitta, al'adun tantanin halitta, enzyme asichements inda PROCEMELINCELES inda PROREMELS yake da mahimmanci. Ikon da ta dace da kuma jituwa tare da tsarin nazarin halittu ya sanya shi sanannen sanannen yanayi don kiyaye kyakkyawan yanayi ga ayyukan da kuma gamsar da kwayoyin halittu daban-daban.
Kafin amfani da gishirin sodium gishiri a kowane takamaiman aikace-aikace, yana da mahimmanci don sake nazarin shawarwarin, shawarwarin shawarwarin masu kaya, da inganta taro da kuma dangane da takamaiman bukatun gwaji.

Roƙo

Hepes sodium gishiri sami aikace-aikace na yaduwa a cikin nazarin halittu da biochemical. Wasu daga cikin mabuɗin amfani sun hada da:
Al'adun tantanin halitta:Ana yawan ƙara yawan gishirin sodium da kafofin watsa labaru don kula da ingantacciyar hanyar PH a cikin kewayon da suka dace don sel don girma da haɓaka.
Wakilin Buff:Ana amfani da gishirin sodium na yau da kullun a matsayin wakili na biyan kuɗi a cikin masu bufforers da mafita, gamsarwa enzyme, furotin enzyme, kuma dabarun enzyme. Yana taimakawa wajen kiyaye ph ta hanyar tsayayya da canje-canje a acidity ko alkaliniti.
Electrophoreses: Ana amfani da gishiri na gishiri a matsayin mai buffer a cikin gel electrophoresis don kula da tsayayyen ph yayin rabuwa da acid na nucleic ko sunadarai. Yana hana juyawa a cikin pH wanda zai iya shafar ƙaura da tsarin rabuwa da kwayoyin a cikin gel.
Kwanciyar hankali An ƙara gishirin sodium a cikin mafita na furotin don magance tsarin su kuma kula da aikinsu. Yana taimakawa wajen kiyaye mafi kyawun yanayin ph Dole ne don kwanciyar hankali da aiki.
Aikin Enzyme: Ana amfani da gishiri na gishiri a matsayin mai buffer a cikin tsoffin halayen enzymatic don kula da ph don ingantaccen aiki na enzyme. Yana taimaka tabbatar da cewa aikin enzymes a mafi girmansu da daidaito.
Hoto na live:A sau da yawa gishiri gishirin yawanci an haɗa shi cikin hanyar da ke kwaikwayon Middia don gwaje-gwajen rayuwa. Ikwircin da ta cika yana taimakawa wajen kula da ph da ake so kuma yana hana hawa hawa da zai iya shafar da sha'awar sel.
Tsarin ilimin halittar kwayoyin halitta: HEPES sodium salt is widely employed in various molecular biology techniques, including DNA or RNA isolation, PCR, DNA sequencing, and protein analysis. Karfin gudummawa yana ba da damar sakamako mai ingantaccen kuma ingantaccen sakamako yayin waɗannan hanyoyin.
Yana da mahimmanci a lura cewa takamaiman aikace-aikacen da kuma maida hankali ne na hepes sodium gishiri na iya bambanta dangane da gwajin gwaji da tsarin nazarin halittu. Sabili da haka, yana da kyau a nemi shaidar da masu siye da kaya don ingantaccen amfani.