Hepes; c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c cast

Hepes; c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c c cast

A takaice bayanin:

Sunan sunadarai:Hepes
CAS No.:7365-45-9
Tsarin kwayoyin halitta:C8h18n2o4s
Nauyi na kwayoyin:238.308
Lambar HS.:Wasara
Fayil na Mol:7365-45-9.mol

Cikakken Bayani

Tags samfurin

Kwatanci: 1- [4- (4- (2- (2- (2-hydroxyethyl) -1-piperazinyl] ethane-2-hydroxyeicacyl) piperazinyhyl) butperazine-1- (2-hyhroxine-1- (2-hyhoresulthonic acid); n- (2-hydroxyethyl) butperazine-n'-2 ethanesulnic acid; n- (2-hyhroxyethyl) butperazine-n- (2-ethanesultfonic acid); N'-2-Hydroxyethylperazine-n-2-ethanesultfonic acid, 4- (2-acixeethonic acid, 4- (2-hydroxyethyl) -;

Kayan guba na hepes

Bayyanar / launi: Farin Crystalline foda
● Trapher matsa lamba: 0pa a 25 ℃
Point ● Melting Points: 234-238 ° C
Index index: N20 / D 1.339
● Matsakaicin tafasa: 408 ℃ [a 101 325 pa]
● Poka: 7.5 (a 25 ℃)
● PSA:89,46000
Bensity: 1.325 g / cm3
● Laja: -0,59930

● Stage Templ.: 7
● Sulquility.:h2o: 1 m A 20 ° C, Share, mai launi
● Sallowararrun ruwa
● Xlogp3: -4.1
● Hydrogen Bond Donor Kidaya: 2
● Hydrogen Bond Karatun: 5
Tasirin Bond: 4
● daidai taro: 238.0987283
● Kididdige Terom Count: 15
● Hadadewa: 254

Bayanin Safya

● Picogram (s):Xi
Lambobin Hadiki: Xi
● Bayani: 36/37/38
Bayanin Tsaro: 24 / 25-22-36-26

M

Canonical murmushi:C1cn (CC +] 1ccs (= o) (= o) [O -]) CCO
Bayanin:An bayyana Hepes a matsayin daya daga cikin mafi kyawun duka-manufar da ke fama da binciken binciken nazarin halittu. A Halitse PH, kwayoyin suna zwitteronic, kuma suna tasiri a matsayin mai buffer a PH 6.8 zuwa 8.2 (Pka 7.55). Ana amfani da shi yawanci a al'adun tantanin halitta a maida hankali tsakanin 5mm zuwa 30 mm. Anyi amfani da Hepes a cikin shirye-shiryen aikace-aikace da yawa, gami da al'adun nama. Ana yawanci amfani dashi don samar da kayan kwayar halitta a cikin iska. Hepes ya sami amfani da shi a cikin gwaje-gwajen Vitro akan MG.
Amfani:Mara guba ga tantanin halitta. Ana amfani dashi azaman mai buffer hydrogen, wanda zai iya sarrafa kewayon PR na dogon lokaci. A maida hankali ne 10-50mmol / l. Gabaɗaya, 20mmol / lheepes a cikin mafita mai gina jiki na iya samun ikon buffer. Buffer nazarin halittu. Hepes gama gari ne na yau da kullun game da kimiyyar halittu, musamman da aka yi amfani da shi cikin al'adun tantanin halitta don kula da ilimin ph. Yana aikata kayan da ake amfani da shi, wanda ake amfani dashi a cikin shirye-shiryen buffers. An bayyana shi a matsayin ɗaya mafi kyawun manufar da aka yi don amfani da shi a cikin ma'aunin ƙwayar cuta.

Cikakken gabatarwar

Hepestsaye ga 2- (4- (2-hydroxyethyl) bututun-2-hydroxin-1-yl) Ethanesulfonic acid. Wakili ne wanda ake amfani dashi a cikin binciken nazarin halittu da biochemical. Hepes yana taimakawa wajen tabbatar da ingantacciyar ph a cikin al'adun al'adun tantanin halitta, saboda yana da ikon duka yarda da ba da gudummawa, don haka yana canzawa canje-canje na ph. Tsarin sunadarai da kaddarorin sa yana da tasiri sosai wajen kiyaye kullun da kuma ingantattun halayyar halittu da tsoffin halayen halittu. An yi amfani da Hepes ɗin da ake amfani da shi a al'adun tantanin halitta, tsarkakakkiyar tsarkin furotin, da dabarun ilimin halittar kwayoyin halitta.

Roƙo

Hepes yana da aikace-aikace da yawa a filayen bincike daban-daban. Wasu daga cikin aikace-aikacen gama gari na Hepes sune:
Al'adun tantanin halitta: An yi amfani da Hepes ɗin a matsayin wani ɓangaren ɓangaren a al'adun sel mai jarida don kula da madaidaiciyar yanayin. Yana taimaka wajen tsara canje-canje ph wanda aka haifar da ayyukan rayuwa da kuma tara kayayyakin sharar gida a cikin kwayoyin halitta.
Enzyme Hards:Hepes ana amfani dashi azaman wakili mai cike da gudummawa a enzyme amintacce don kula da ph, wanda yake da mahimmanci don aikin enzymes. Yana taimaka tabbatar da tabbaci da sakamako na haifuwa.
Electrophoreses:Ana amfani da Hepes azaman wakili na Bufendin a cikin dabarun lantarki kamar polyacrylamai gel electrophoresis (shafi) da kuma agarose gel electrophoresesis. Yana taimakawa wajen kula da mafi kyawun PH don mafi kyawun rabuwa da bincike game da macromearcolecules kamar su sunadarai da nucleic acid.
Tsarkakewar Tsaro:Hepes wani lokaci ana cikin wasu lokuta a cikin tsarkakewar fursunoni don kula da ph da ake so yayin matakai daban-daban na aiwatarwa. Yana taimakawa wajen tabbatar da kwanciyar hankali da ayyukan sunadarai yayin matakan tsarkakewa.
Tsarin ilimin halittar kwayoyin halitta:Hepes za a iya amfani da su a cikin dabaru na kwayoyin halitta daban-daban, kamar PCR (polymorres sarkar amsawa) da DNA SEQUCTE, don kula da m ph. Yana taimaka wajen inganta aikin enzymes da tabbatar da sakamako mai gamsarwa.
Yana da mahimmanci a lura cewa takamaiman taro na hepes da aikace-aikacen sa na iya bambanta dangane da yanayin gwaji da buƙatun.